affinity chromatography

A new methodology for preparation of ovine LH with high purity by anti-oLH_ β-IgG immuno-affinity chromatography has been reported. The biological activity of oLH is corresponding to 3,050 hCG IU/ mg, exhibiting a single band in PAGE.

本文报道用抗oLHβ-IgG免疫亲和层析柱纯化oLH的新方法，得到了生物学活性相当于3050·hCG IU/mg的oLH精制品，其电泳图谱显示高纯度的单一色带。

The fusion protein was purified through Nickel-affinity chromatography column.

用镍柱纯化融合蛋白.

Purification by Affinity Chromatography and Characterization of Tea Leaf β-Glucosidase

茶叶β-葡萄糖苷酶亲和层析纯化与性质研究

The purification of AFP from the cancered ascites by means of affinity chromatography and the preparation of corresponding monoclonal antibody

从癌性腹水中亲和层析提纯AFP并制备相应的单克隆抗体

The polypeptide of interest was purified by Ni2+ affinity chromatography under denaturing conditions, followed by renaturation of the recombinant protein by dialysis, and digestion with enterokinase to release the chimeric peptide.

经表达条件优化，大量制备重组蛋白，变性条件下进行镍离子亲和层析纯化。对纯化后的重组蛋白进行复性，用肠激酶切割复性蛋白以获得目的多肽。

Affinity chromatography is one of the most important tools in protein research.

亲和层析技术是目前重要的蛋白质研究技术之一.

Lectins in haemolymph of Sarcophaga peregrina larvae and pupa were purified by affinity chromatography.

用亲和层析法纯化了棕尾别麻蝇幼虫和蛹血淋巴凝集素.

The supernant flows through Ni-NTA gel for affinity chromatography, and thepurity is over 91.5 %.

上清经Ni-NTA凝胶亲和层析, 纯度达到91.5%以上.

The expressed product issoluble, MW 18 kD , which was purified by Ni + metal chelate affinity chromatography.

表达产物为可溶性蛋白,分子量约为18kD,通过Ni~+离子亲和层析一次性纯化.

One step Purification of Tissue type Plasminogen Activator Derived from Expression by Milk Gland of Transgenic Goat Using Affinity Chromatography

应用一步亲和层析法纯化转基因山羊乳腺定位表达的组织型纤维蛋白溶酶原激活剂

METHODS BALB/ c mice were immunized with human liver microsome proteins, and mAb was prepared by hybridoma technique. The crude mAb was purified by protein-G affinity chromatography.

方法：以含hCE-Ⅱ的人肝脏微粒体蛋白混合抗原免疫BALB/c小鼠，采用杂交瘤技术制备鼠抗hCE-Ⅱ的mAb，并用Protein-G亲和层析法纯化mAb。

CALB was purified through Ni-NTA affinity chromatography and ultrafiltration, and was then analyzed by SDS-PAGE. The result showed that the CALB protein expressed by P. pastoris was about 35 kDa, which might contain the glycosylated brach chain.

对经Ni-NTA亲和层析和超滤浓缩纯化的CALB进行SDS-PAGE分析，发现在CalB基因在P.pastoris中表达产物的大小为35kDa（含糖基化侧链）。

Furthermore, the protein isolated in the periplasm can be purified by nickel affinity chromatography.

而且从周质空间分离的蛋白经过镍住得到了进一步的纯化.

Methods: These hormones were extracted and purified from cooled human pituitary glands by affinity chromatography 、 gel filtration chromatography and so on.

方法：以冷冻人脑垂体为原料经离子交换层析、凝胶过滤和亲和层析等方法可获得达国际标准品水平的激素纯品。

After denature and renature procedure in Ni~ ( 2+)_NTA affinity chromatography column, soluble chitinase was obtained.

在Ni2+-NTA亲和柱上经变、复性和纯化，得到可溶的几丁质酶。

Purification of human red blood cell Group-A protein by affinity chromatography

人红细胞血型A抗原的亲和层析纯化

Made sheep anti? human IgG Fab Sepharose 4 B affinity chromatography, purified the souble Fab fragment.

制备羊抗人 IgGFab 抗血清、建立抗人IgGFab抗体 -Sepharose4B亲和层析柱, 纯化Fab片段.

Purpose To study purification method of the low molecular weight heparin ( LMWH ) by affinity chromatography.

目的探索亲和色谱纯化低分子 肝素 的方法.

Analysis to Fields Synergy for the Affinity Chromatography in a Tapered Bed

锥形床亲和层析过程的场协同分析

Affinity chromatography used to play important rule in the separation and purification of biomacromolecule.

亲和色谱多用于生物大分子的分离与纯化.

Purified vMIP ⅱ was produced through metal chelated affinity chromatography, enterokinase digestion to free vMIP ⅱ from fusion protein and cation exchange chromatography.

经金属离子亲和吸附、肠激酶消化以游离vMIPⅡ、阳离子交换层析等步骤纯化目的蛋白。

Study on isolating complement C3d from the chicken serum by the method of immuno affinity chromatography

免疫亲和层析法分离鸡补体C3d蛋白研究

Synthesis of Non-porous Microsphere of Poly ( p-Phenylenterephthalamide) ( PPTA) Used as the Matrix of Affinity Chromatography Stationary Phase

聚苯酰亚胺树脂非多孔微球亲和色谱固定相基体的合成

Expressed proteins as inclusion bodies were solubilized , refolded and purified by GST affinity chromatography.

表达产物为包涵体形式,溶解和折叠后,以GST亲合层析色谱纯化.

Hepatitis delta antigen ( HDAg) from the liver of the woodchuck hepatitis virus ( WHV) carrier ( woodchuck infected with HDV) was purified through Sepharose 4B column and affinity chromatography column.

用丁型肝炎病毒（HDV）感染慢性携带土拨鼠肝炎病毒的东方土拨鼠（Woodchuck）后，取其肝组织制成匀浆，离心后经Sepharose4B柱，免疫亲和层析柱，获得高丰度的HDAg。

Recombinant hKS with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

Isolation and purification of human cardiac troponin ⅰ using affinity chromatography

亲和层析法分离纯化人心肌肌钙蛋白Ⅰ

Recombinant human-like collagen withattacked to the carboxyl be easily purified by Metal Chelated Affinity Chromatography ( MCAC ) .

研究了应用金属螯合亲和 层析法 纯化重组类人胶原蛋白的条件.

Objective To investigate the effect of position of hexahistine in recombinant protein on nickel affinity chromatography.

目的研究六聚组氨酸在重组蛋白中的位置对镍亲和层析的影响.

A lectin from the edible tomato ( Lycopersicon esculentum) was purified by hen ovomucoid-Sepharose 4B affinity chromatography.

番茄成熟果实经抽提上鸡卵类粘蛋白（OM）-Sepharose4B亲和层析柱分离纯化制得番茄凝集素。